Development of glasses doped with therapeutic and biocide ions for tissue engineering has accelerated in recent years. Tissue injury as a result of the implantation of a foreign body or traumatic event activates the body's defensive system reactions (e.g. acute or chronic infection). Complex studies and in vitro tests are required for the selection of proper action of biocide ions (e.g. Cu, Ag) to suppress the infection shortly after a traumatic event or to enable continuous healing and antibacterial effect for a longer period of time. Mesoporous silicate glass doped with Cu-Sr/Mg ranging from 0.5 mol% to 4 mol% was tested to determine the rate at which biocide ions are released into the solution with pH(7.4). A method of direct monitoring of the concentration of ions released from the glass under dynamic conditions by atomic emission spectrometry (ICP-OES) was developed as the most suitable way of monitoring the immediate (e.g. burst) chemical response of the glass in contact with an aqueous medium. The highest leaching rate and amount of dissolved copper were measured for the Cu and Cu/Sr mesoporous glass with CuO content up to 1 mol%. An increase of CuO concentration in Cu/Sr and Cu/Mg co-doped mesoporous glasses up to 2 mol% resulted in a marked increase of the size of the inhibition zone for S. aureus and E.coli bacteria compared to mesoporous calcium-silicate glass without any dopant. The antibacterial effect and steady continuous release of copper observed for these glass systems could be beneficial for applications requiring continuous suppression of persistent inflammation. 

Acknowledgment:
This item is a part of dissemination activities of project FunGlass. This project has received funding from the European Union´s Horizon 2020 research and innovation programme under grant agreement No 739566. The authors would like to acknowledge VEGA 01-0191-20 grant.