This study aims at determining the cortisol dosage in saliva samples using mesoporous titanium oxide and complex impedance spectroscopy. A special gold interdigitated electrode (IDE) platform of semiconducting mesoporous barium titanate oxide nanoparticles (TiBaO3) was developed. To increase the cortisol binding specificity in the complex samples, albumin was attached on mesoporous surface particles as a surface anti-binder agent. In the electrochemical cell construction, the IDE was used as a support to the TiBaO3 power deposits. Different analytical cortisol standard samples were prepared and measured at a constant voltage of 5 mV; the nominal current of 40 nA was generated at 1 kHz. The complex impedance was recorded using a sinusoidal input voltage in the frequency range of 100 Hz to 2 kHz. Principal component analysis (PCA) was used for data processing. Cortisol levels as low as 40 nanograms per milliliter were detected. Further studies are being conducted to use this method to quantify the cortisol in complex human saliva samples.